Coding

Part:BBa_K2108001:Design

Designed by: Maya de Vos   Group: iGEM16_NorthernBC   (2016-10-14)


YiaT-MCS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 641
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 641
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 790
    Illegal XhoI site found at 784
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 641
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 641
    Illegal NgoMIV site found at 325
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We ensured that the multiple cloning site did not contain any restrictions sites that were also contained in the prefix or suffix.


Source

The truncated YiaT sequence was derived from: Han, M.J., Lee, S.H. (2014). An efficient bacterial surface display system based on a novel outer membrane anchoring element from the Escherichia coli protein YiaT. FEMS Microbiology Letters, 362, 1-7.

References